Journal: Cell reports

Article Title: CBX7 Induces Self-Renewal of Human Normal and Malignant Hematopoietic Stem and Progenitor Cells by Canonical and Non-canonical Interactions.

doi: 10.1016/j.celrep.2019.01.050

Figure Lengend Snippet: Figure 1. CBX7 Overexpression Induces Enhanced Long-Term Engraftment, Myelopoiesis, and Self-Renewal of Primitive CD34+CD38 HSPCs In Vivo (A) Human chimerism levels in the peripheral blood of NSG mice upon transplantation of 200,000 CD34+GFP+CBX7-overexpressing or empty vector (EV) control cord blood cells. (B) Primary recipients were sacrificed after 28–40 weeks, and human engraftment in bone marrow was evaluated. (C) Human chimerism levels in the peripheral blood of NSG mice upon transplantation of 7 days ex vivo-cultured CBX7-overexpressing or empty vector control CD34+ cord blood cells. (D) Human engraftment in bone marrow of mice shown in (C), combined analysis of mice sacrificed after 18, 22, and 33 weeks post-transplant. (E) Relative engraftment of human CD19+, CD3+, and CD33+ cells within human CD45+GFP+ bone marrow cells of mice shown in (C), combined analysis of mice sacrificed after 18, 22, and 33 weeks post-transplantation. (F) Human myeloid engraftment in bone marrow of mice shown in (C), combined analysis of mice sacrificed after 18, 22, and 33 weeks post-transplantation. (G) Frequency of CD34+CD38 cells in huCD45+GFP+lin-CD34+ cells in bone marrow of mice shown in (C), 18 combined analysis of mice sacrificed after 18, 22, and 33 weeks post transplantation. Identically colored circles indicate 3 paired experimental and control samples that originate from the same cord. Statistical analysis was performed using a two-tailed Mann-Whitney test.

Article Snippet: N/A Oligonucleotides See Table S8 N/A Software and Algorithms Graphpad Prism (v5-7) Graphpad Prism https://www.graphpad.com ELDA (Hu and Smyth, 2009) http://bioinf.wehi.edu.au/software/elda/ FlowJo Version X.0.7 Other CD34+ MicrobeadKit Miltenyi Biotec 130-056-702 Lymphoprep Stem cell technologies 7861 LS Columns Miltenyi Biotec 130-042-401 e2 Cell Reports 26, 1906–1918.e1–e8, February 12, 2019

Techniques: Over Expression, In Vivo, Transplantation Assay, Plasmid Preparation, Control, Ex Vivo, Cell Culture, Two Tailed Test, MANN-WHITNEY